AICAR 50 mg

AICAR 50 mg — the standard AMPK activator in metabolic cell biology.
AICAR is not a peptide but a nucleoside analogue, and it has become one of the single most-used tool compounds in cellular energy research. If a paper investigates AMPK, there is a strong chance AICAR appears in the methods. Supplied as a high-content 50 mg vial for laboratories running sustained culture work.

What is AICAR?

AICAR (5-aminoimidazole-4-carboxamide ribonucleotide, also known as acadesine) is a purine nucleotide intermediate found naturally in the de novo purine biosynthesis pathway. Inside the cell it is phosphorylated to ZMP, a compound that mimics the structure of AMP closely enough to be recognised by AMP-activated protein kinase — the cell's central energy sensor. This makes AICAR a direct pharmacological entry point into AMPK signalling, which sits upstream of glucose uptake, fatty acid oxidation, mitochondrial biogenesis and mTOR regulation. Its mechanism is unusually well defined for a metabolic tool compound, which is precisely why it has become the field's default activator.

Research interest

• AMPK activation — the reference pharmacological activator in cellular energy-sensing studies.
• Glucose uptake — investigated in muscle and adipocyte models examining transporter translocation.
• Fatty acid oxidation — studied for its role in substrate-switching and lipid metabolism research.
• Mitochondrial biogenesis — of interest to researchers examining endurance-associated adaptation pathways.
• mTOR pathway crosstalk — applied in work mapping the AMPK–mTOR regulatory interface.

Specifications

• Content: 50 mg of active substance per vial
• Form: Lyophilised powder (sealed vial)
• Purity: Pharmaceutical-grade, batch-tested
• Storage: Sealed, dry and dark at 2–8 °C; −20 °C for extended storage

Handling & storage

As a nucleoside analogue, AICAR is comparatively robust in the dry state but should still be protected from moisture, which drives hydrolytic degradation. Keep the vial sealed and cold, and allow it to warm to ambient temperature before opening so that condensation does not reach the powder. Reconstitution should follow the receiving laboratory's validated protocol and the solvent system appropriate to the intended assay; dissolve fully and confirm a clear solution before use. Because cell-culture work typically consumes a 50 mg vial over many experiments, plan the aliquot scheme first — stock solutions held frozen in single-use volumes give far better run-to-run consistency than a repeatedly thawed master stock.

Why source from Rogue

• Verified purity — batch-tested material with documented identity and assay results.
• European supply chain — factory-sealed vials held in controlled conditions, never repackaged or grey-market.
• Discreet, tracked delivery — rapid EU-wide dispatch in unbranded, protective outer packaging.

For research purposes only. Not a medicinal product. Not for human or veterinary consumption. Intended for qualified laboratory research use only.

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